Satb2通过调控Selenbp1在放射性结肠炎中的发挥保护作用的机制研究-豆柴文库

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Satb2通过调控Selenbp1在放射性结肠炎中的发挥保护作用的机制研究
Title:MechanismsofSATB2-mediatedProtectioninRadiation-inducedColitisviaSelenbp1Regulation
Abstract:
Radiation-inducedcolitis(RIC)isacommonandseverecomplicationofpelvicradiotherapy.ThemolecularmechanismsunderlyingtheprotectiveeffectsofspecialAT-richsequence-bindingprotein2(SATB2)inRICarepoorlyunderstood.ThisstudyaimedtoinvestigatethemechanismsthroughwhichSATB2exertsitsprotectiveeffectsinRICviatheregulationofselenoproteinP1(Selenbp1).
1.Introduction:
Radiationtherapyisaneffectivetreatmentforvariouspelvicmalignancies.However,itcanleadtoradiation-inducedcolitis,characterizedbyinflammation,tissuedamage,andimpairedgutfunction.SATB2,anuclearproteininvolvedinchromatinremodelingandgeneregulation,hasbeenshowntohaveprotectiveeffectsindifferentdiseasemodels.Selenbp1,aselenocysteine-containingprotein,playsavitalroleinmaintainingseleniumhomeostasis,anditsdysregulationhasbeenimplicatedininflammatorydiseases.ThisstudyaimedtoelucidatetheroleofSATB2inRICandexploreitsinteractionwithSelenbp1.
2.Methods:
2.1Animalmodel:MaleC57BL/6micewereexposedtopelvicirradiationtoinducecolitis.
2.2Tissueanalysis:Colonictissueswerecollectedatdifferenttimepointsafterirradiationandassessedforhistopathologicalchanges,oxidativestressmarkers,andinflammatorycytokinelevels.
2.3SATB2knockdown:Inaseparateexperimentalgroup,micewereinjectedwithlentiviralparticlescarryingSATB2shRNAtoinvestigatetheeffectsofSATB2knockdownonRICseverity.
2.4SATB2overexpression:AnotherexperimentalgroupreceivedlentiviralparticlescarryingSATB2overexpressionvectorstoassesstheprotectiveeffectsofSATB2onRIC.
2.5Mechanisticstudies:
a.RNA-Seqanalysis:ColonictissueRNAsamplesweresubjectedtoRNA-SeqtoidentifygenesdifferentiallyregulatedbySATB2inRIC.
b.Westernblotting:ProteinexpressionlevelsofSelenbp1andotherrelevantmarkerswereanalyzedincolonictissues.
c.Immunofluorescence:Co-localizationstudieswereperformedtoexaminethedistributionofSATB2andSelenbp1incolonictissues.
3.Results:
3.1SATB2expressionwasupregulatedin