壳聚糖-透明质酸支架用于低氧靶向性抗癌药物筛选的研究-豆柴文库

您所在位置：网站首页 / 壳聚糖-透明质酸支架用于低氧靶向性抗癌药物筛选的研究.docx / 文档详情

在线预览结束，喜欢就下载吧，查找使用更方便

5 金币

下载文档

/ 2

下载提示

如果您无法下载资料，请参考说明：

1、部分资料下载需要金币，请确保您的账户上有足够的金币

2、已购买过的文档，再次下载不重复扣费

3、资料包下载后请先用软件解压，在使用对应软件打开

文本预览

壳聚糖-透明质酸支架用于低氧靶向性抗癌药物筛选的研究
Title:StudyontheUseofChitosan-HyaluronicAcidScaffoldforTargetedAnticancerDrugScreeningunderHypoxicConditions
Abstract:
Cancerremainsasignificantglobalhealthissue,andcurrentdrugscreeningmethodsoftenfailtoaccuratelypredicttheefficacyofdrugsunderhypoxicconditions.Therefore,itiscrucialtodevelopnewstrategiesfortargetedanticancerdrugscreening.Thisstudyaimstoinvestigatethepotentialofusingachitosan-hyaluronicacidscaffoldforthispurpose.
Introduction:
Hypoxia,orlowoxygenlevels,isacommoncharacteristicofsolidtumors.Itcreatesahostilemicroenvironmentthatcanlimittheeffectivenessoftraditionalchemotherapydrugs.Therefore,thereisaneedtodevelopnewdrugscreeningmethodsthatconsiderthehypoxicconditionswithintumors.Inrecentyears,biomaterialscaffoldshavegainedattentionasapromisingtoolfordrugscreeningduetotheirabilitytomimicthenativetumormicroenvironment.Amongthesescaffolds,chitosanandhyaluronicacidhavereceivedparticularinterestduetotheirbiocompatibleandbiodegradableproperties.
Methods:
Firstly,chitosan-hyaluronicacidscaffoldswerepreparedusingasimpleandreproduciblemethod.Thescaffold'sphysical,chemical,andmechanicalpropertieswerecharacterizedtoensureitssuitabilityfordrugscreeningapplications.Additionally,thescaffold'scapacitytomaintainanappropriatehypoxicenvironmentwasevaluated.
Next,apanelofanticancerdrugswithdifferentmechanismsofactionwasselectedtostudytheireffectivenessunderhypoxicconditions.Thesedrugsweretestedindividuallyandincombinationusingthechitosan-hyaluronicacidscaffoldandconventionalscreeningmethods,suchas2Dcellcultureandhypoxiachambers.Theviability,proliferation,anddrugresistanceofcancercellswereassessedusingvariouscellularassays.
Results:
Thechitosan-hyaluronicacidscaffoldshowedexcellentbiocompatibilityandbiodegradability.Itadequatelymaintainedhypoxicconditionswithinthescaffold,mimickingthetumormicroenvironment.Furthermore,thescaffoldprovideda3Dcultureplatformthatbetterrepresentedthecomplexityoftumortissuescomparedtotraditional2Dcellculturemethods.
When