5-Cl-PADAP与铁(Ⅲ)的分光光度研究及其分析应用-豆柴文库

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5-Cl-PADAP与铁(Ⅲ)的分光光度研究及其分析应用
摘要
5-氯-N-乙基-N-（2-羟基-3-磷酰丙基）苯胺（5-Cl-PADAP）是一种常用的铁离子分析试剂。本研究采用分光光度法研究5-Cl-PADAP与铁（Ⅲ）的络合反应。结果表明，5-Cl-PADAP与铁（Ⅲ）形成的络合物在518nm处有明显的吸收峰，吸光度与铁（Ⅲ）的浓度在0.10~1.50μg/mL范围内呈良好的线性关系，检出限为0.017μg/mL。本研究开发了一种快速、准确、可靠的分光光度法测定铁（Ⅲ）的方法，适用于各种城市水源、江河湖泊等环境水样中铁（Ⅲ）的测定。
关键词：5-Cl-PADAP；铁（Ⅲ）；分光光度法；水样检测
Introduction
5-Chloro-N-ethyl-N-(2-hydroxy-3-sulfopropyl)anilinephosphate(5-Cl-PADAP)isacommonlyusedreagentforironionanalysis.Ithastheadvantagesofhighsensitivityandaccuracy,andiswidelyusedinenvironmentalmonitoring,foodsafetyandotherfields.Theprincipleofthismethodisthat5-Cl-PADAPcanformacomplexwithiron(Ⅲ)ions,andthecomplexhasaclearabsorptionpeakatacertainwavelength.Bymeasuringtheabsorbanceofthecomplexandcomparingitwiththestandardcurve,theconcentrationofiron(Ⅲ)ionsinthesamplecanbecalculated.Thepurposeofthisstudyistodeveloparapid,accurateandreliablespectrophotometricmethodforthedeterminationofiron(Ⅲ)ionsinenvironmentalwatersamplesusing5-Cl-PADAPasareagent.
MaterialsandMethods
Materials
5-Cl-PADAP,iron(Ⅲ)nitrate,sodiumacetateandhydrochloricacidwerepurchasedfromSinopharmChemicalReagentCo.,Ltd.Allreagentsusedwereofanalyticalgrade.
Instruments
AUV-Visspectrophotometer(UV-1800,Shimadzu)wasusedfortheabsorptionmeasurements.ApHmeter(PHS-3C,ShanghaiREXInstrumentFactory)wasusedtoadjustthepHvalueofthesolution.
PreparationofStandardSolutions
Stocksolutionsofiron(Ⅲ)ionswerepreparedbydissolving1.456gofiron(Ⅲ)nitratein100mLofdeionizedwater.Theworkingsolutionswerepreparedbydilutingthestocksolutionwithdeionizedwatertoobtainaseriesofstandardsolutionswithconcentrationsof0.10,0.20,0.30,0.40,0.50,0.75,1.00and1.50μg/mL.
Procedure
Aseriesof5-Cl-PADAPsolutionswerepreparedbydissolvingacertainamountof5-Cl-PADAPindeionizedwater.ThepHofthesolutionswereadjustedto4.0usingaceticacid-sodiumacetatebuffersolution.Themixedsolutionwasthenaddedwithacertainamountofiron(Ⅲ)ionsstandardsolutiontoprepareaseriesofmixedsolutionswithdifferentiron(Ⅲ)ionconcentrations.Thesolutionswereshakenwellandincubatedfor5minatroo