烫伤大鼠腹腔巨噬细胞释放细胞因子作用的研究-豆柴文库

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烫伤大鼠腹腔巨噬细胞释放细胞因子作用的研究
[Abstract]
Thepurposeofthisstudyistoinvestigatethereleaseofcytokinesbyperitonealmacrophagesinratsafterburninjury.Inthisstudy,weexploredthechangesinthecytokinelevelsintheperitonealmacrophagesofratsafterburninjury,inordertoprovideatheoreticalbasisforthestudyofburninjuryandassociatedimmuneresponses.
[Keywords]
Burninjury,peritonealmacrophages,cytokines
[Background]
Burninjuryisacommontypeoftraumathatcanleadtosignificanttissuedamageandsystemicinflammatoryresponsesyndrome(SIRS).Peritonealmacrophagesplayacriticalroleintheimmuneresponsetoburninjury,releasingcytokineswhichcanleadtoinflammationandimmunesystemactivation.Previousstudieshaveshownthatthecytokinelevelsareelevatedintheperipheralbloodofburnpatients,butthemechanismsunderlyingthesechangesremainunclear.
[Methods]
MaleWistarratsweighing220-250gwererandomlydividedintoacontrolgroupandaburninjurygroup(n=10each).Theburninjurygroupwassubjectedtoa30%totalbodysurfacearea(TBSA)scaldburnthroughhotwaterimmersionoftherats'shavedbacksfor10s.Thecontrolgroupreceivedashamburnbyimmersioninroomtemperaturewaterfor10s.After24h,cellswerecollectedfromtheperitonealcavitybylavagewithsterilesaline.Thecellswerethenculturedinvitrofor24h.Thecytokinelevelsinthesupernatantweremeasuredusingenzyme-linkedimmunosorbentassay(ELISA).Thelevelsofcytokinesincludingtumornecrosisfactor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6),andinterleukin-10(IL-10)weremeasured.
[Results]
Comparedtothecontrolgroup,thelevelsofTNF-α,IL-1β,andIL-6weresignificantlyincreasedintheperitonealmacrophagesofratsafterburninjury(p<0.01).Meanwhile,thelevelofIL-10wassignificantlydecreased(p<0.05).Theseresultsindicatethatburninjuryleadstoaninflammatoryresponseintheperitonealmacrophagesofrats,withincreasedproductionofpro-inflammatorycytokinesanddecreasedproductionofanti-inflammatorycytokines.
[Conclusion]
Inconclusion,ourstudydemonstratesthatburninjuryinduceschangesincytokinelevelsinperitonealmacrophagesinrats,resultinginaninflammatoryresponsewithincreasedpro