杀虫剂对抗药性家蝇的兴奋性研究-豆柴文库

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杀虫剂对抗药性家蝇的兴奋性研究
摘要：
药剂抗性是家蝇控制的一个主要挑战。为了探讨杀虫剂对抗药性家蝇的兴奋性，本文设计了实验，测量了抗药性家蝇在杀虫剂处理下的运动活性和神经元活性。结果表明，杀虫剂处理显著提高了抗药性家蝇的运动活性和神经元活性。这一研究为家蝇控制提供了一种新的思路和方法。
关键词：抗药性家蝇；杀虫剂；兴奋性；神经元活性
Introduction
Thehousefly(Muscadomestica)isacommonpestthatisfoundworldwide.Itisknownforitsabilitytocarryandspreaddisease-causingmicroorganisms.Oneofthemainchallengesincontrollinghousefliesisdrugresistance.Thedevelopmentofinsecticideresistanceisamajorconcerninthecontrolofhouseflies.
Theuseofinsecticidestocontrolpestsisacommonpractice.However,itisknownthattheapplicationofinsecticidescanleadtothedevelopmentofresistanceinpests.Therearedifferenttypesofresistance,includingmetabolicresistanceandtarget-siteresistance.Inmetabolicresistance,thepestdevelopsstrategiestodetoxifyandeliminatetheinsecticidefromitsbody.Intarget-siteresistance,thepestdevelopsmutationsinthetargetsiteoftheinsecticide.
Thedevelopmentofdrugresistancehasbeenstudiedextensivelyinthehousefly.Ithasbeenreportedthatthereisahighprevalenceofinsecticideresistanceinhouseflies.Theresistanceiscausedbygeneticmutationsthatoccurinthetargetsitesoftheinsecticides.Insecticideresistanceinhousefliesisamajorthreattothecontrolofthispest.
Inthisstudy,weinvestigatedtheeffectofinsecticidesontheexcitabilityofdrug-resistanthouseflies.Wehypothesizedthatinsecticideswouldincreasetheexcitabilityofdrug-resistanthouseflies.
Methods
Weusedapopulationofhousefliesthathadbeenexposedtoinsecticidesforalongperiodoftime.Theflieshaddevelopedresistancetotheinsecticides.Wedividedthefliesintotwogroups.Onegroupwastreatedwithaninsecticide,andtheothergroupwasnottreated.Theinsecticidethatwasusedinthisstudywasmalathion.
Wemeasuredthelocomotoractivityofthefliesusingavideotrackingsystem.Thesystemrecordedthemovementofthefliesinacirculararena.Wemeasuredthedistancetraveledbythefliesinaminute.Wealsorecordedthenumberoftimesthefliescrossedthearena.
Wemeasuredtheneuronalactivityofthefliesusingpatchclamprecordings.TherecordingsweremadefromtheKenyoncellsofthemushroombody,whichispartoftheinsectbrain.W