牛排菇SB65菌株液体培养基和培养条件的优化-豆柴文库

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牛排菇SB65菌株液体培养基和培养条件的优化
I.Introduction
SB65isatypeofsteakmushroomthathasattractedattentionduetoitshighnutritionalvalueandstrongmedicinalproperties.SB65canbecultivatedinaliquidmedium,allowingforexpandedproductionandcommercialapplications.ThisstudyaimedtooptimizetheliquidmediumandcultureconditionsforthecultivationofSB65toenhanceitsbiologicalactivityandyield.
II.MaterialsandMethods
1.FungalStrain
TheSB65strainwasobtainedfromtheFungalCultureCollectionoftheChinaGeneralMicrobiologyCultureCollectionCenter(CGMCC).
2.CultureMedium
Abasalliquidmediumcomposedofglucose(20g/L),peptone(10g/L),andyeastextract(5g/L)wasusedforSB65liquidculture.
3.OptimizationofCultureConditions
Theeffectofvariouscultureconditions,includingtemperature,initialpH,inoculumsize,andagitationrate,wasinvestigatedtooptimizetheSB65liquidculture.Thefermentationwascarriedoutin500-mLErlenmeyerflaskscontaining100mLofthebasalmedium,whichwasautoclavedat121°Cfor20min.
4.FermentationProcessandSampling
Theinoculatedmediumwasincubatedat25°Cand30°Cfor6dayswithdifferentagitationrates(100rpm,150rpm,and200rpm).SampleswerecollectedonadailybasistoobservethegrowthofSB65.
5.MeasurementofBiomassandExtracellularEnzymesActivity
Themicrobialbiomasswasquantifiedbydryweight,andtheactivityofextracellularenzymes,includingperoxidase,laccase,andcellulase,wasmeasuredusingstandardmethods.
III.Results
1.OptimizationofTemperatureandpH
SB65grewwellat25°Cand30°C,withoptimalmycelialgrowthobservedat25°C.TheSB65mycelialgrowthdecreasedattemperaturesabove30°C,andtheyieldalsodecreasedasaresult.TheinitialpHofthemediumhadasignificanteffectonSB65growth,withoptimalgrowthoccurringatapHof6.5.
2.OptimizationofInoculumSizeandAgitationRate
TheinoculumsizehadasignificantimpactonSB65growth,withamaximumbiomassyieldachievedwithaninoculumsizeof10%.AgitationratealsohadasignificanteffectonSB65growth,withthehighestyieldachievedatanagitationrateof150rpm.
3.EnzymaticActivity
Thelevelofextracellularenzymeactivityvariedwiththefermentationconditions.Atoptimalconditions,t