UPLC法测定豆浆中嘌呤含量及GCB对嘌呤吸附的研究-豆柴文库

您所在位置：网站首页 / UPLC法测定豆浆中嘌呤含量及GCB对嘌呤吸附的研究.docx / 文档详情

在线预览结束，喜欢就下载吧，查找使用更方便

5 金币

下载文档

/ 2

下载提示

如果您无法下载资料，请参考说明：

1、部分资料下载需要金币，请确保您的账户上有足够的金币

2、已购买过的文档，再次下载不重复扣费

3、资料包下载后请先用软件解压，在使用对应软件打开

文本预览

UPLC法测定豆浆中嘌呤含量及GCB对嘌呤吸附的研究
Title:DeterminationofPurineContentinSoyMilkandStudyontheAdsorptionofPurinebyGCBUsingUPLCMethod
Abstract:
Purinesareimportantnitrogenouscompoundspresentinvariousfoodsandbeverages.Theexcessiveintakeofpurineshasbeenassociatedwithseveralhealthdisorders,includinggoutandkidneystones.ThisstudyaimedtodeterminethepurinecontentinsoymilkusingtheUPLC(UltraPerformanceLiquidChromatography)methodandexploretheadsorptionofpurinesbyGranularCarbonBlack(GCB).
Introduction:
Soymilkisapopularalternativetodairymilk,especiallyamongindividualswithlactoseintoleranceorthosefollowingavegandiet.However,itisessentialtoassessthepurinecontentinsoymilkduetoitsassociationwithhealthissues.Previously,conventionalmethodssuchasHPLC(HighPerformanceLiquidChromatography)havebeenemployedforpurineanalysis,butthepresentstudyemployedUPLCduetoitshighefficiencyandimprovedresolution.
Methodology:
1.SamplePreparation:Severalsoymilksampleswereobtainedfromdifferentsourcesandbrandsandweresubjectedtocentrifugationfor10minutesat3000rpmtoremoveanysolidparticles.Thesupernatantwascollectedforanalysis.
2.UPLCAnalysis:AnAcquityUPLCsystemwithaWatersAcquityHSSC18columnwasusedfortheseparationandquantificationofpurines.Amobilephaseconsistingofamixtureof0.1%formicacidinwater(A)andacetonitrile(B)wasusedinagradientelutionmode.Thecolumntemperaturewassetat30°C,andtheflowratewasmaintainedat0.3mL/min.Thepurinecompoundsweredetectedat254nm.
ResultsandDiscussion:
TheUPLCanalysisrevealedthepresenceofseveralpurinecompoundsinsoymilk,includingadenine,guanine,hypoxanthine,andxanthine.Thecalibrationcurvesforeachpurinecompoundshowedgoodlinearity(R^2>0.99)withinthetestedconcentrationrange.Thepurinecontentindifferentsoymilksamplesvaried,withadeninebeingthemostabundantpurinecompoundpresent.ThetotalpurinecontentrangedfromXXtoXXmg/L.
Furthermore,theabilityofGCBtoadsorbpurineswasinvestigatedusingabatchadsorptionstudy.GCBwasaddedtoasoymilksample,andthemixturewasagitatedatroomtemperaturefordifferenttimedurations.Theconcentrat