UPLC-MSMS检测肉制品中16种杂环胺前处理方法筛选.docx

UPLC-MSMS检测肉制品中16种杂环胺前处理方法筛选
Title:ScreeningofPre-processingMethodsfortheDetectionof16HeterocyclicAminesinMeatProductsUsingUPLC-MS/MS
Introduction:
Heterocyclicamines(HCAs)areagroupofcarcinogeniccompoundsthatareformedduringthecookingofmeatandfishathightemperatures.Thesecompoundshavebeenfoundtobemutagenicandhavebeenlinkedtovarioustypesofcancers.Therefore,thedetectionandquantificationofHCAsinmeatproductsisofgreatimportanceforfoodsafetyandconsumerhealth.Ultra-performanceliquidchromatography-tandemmassspectrometry(UPLC-MS/MS)isapowerfulanalyticaltechniquethathasbeenwidelyusedfortheanalysisofsmallmolecules,includingHCAs.However,beforeanalysis,arobustpre-processingmethodisnecessarytoextractandpurifytheHCAsfromthecomplexmatrixofmeatproducts.Inthisstudy,weaimtoscreenandcomparedifferentpre-processingmethodsfortheanalysisof16HCAsinmeatproductsusingUPLC-MS/MS.
Methods:
1.SamplePreparation:
-Differentmeatsamples(e.g.,chicken,beef,andpork)willbeselectedforthisstudy.
-Thesampleswillbehomogenizedanddividedintoaliquotsformethodcomparison.
-Variouspre-processingmethodswillbeevaluated,includingsolid-phaseextraction(SPE),QuEChERS(quick,easy,cheap,effective,rugged,andsafe),andliquid-liquidextraction(LLE).
-DifferentextractionsolventsandconditionswillbetestedtooptimizetherecoveryandsensitivityofthetargetHCAs.
-TheextractedsampleswillbeevaporatedtodrynessandreconstitutedinsuitablesolventsforUPLC-MS/MSanalysis.
2.UPLC-MS/MSAnalysis:
-AUPLCsystemcoupledwithatriplequadrupolemassspectrometerwillbeemployedfortheanalysisofHCAs.
-AsuitableUPLCcolumnandmobilephasecompositionwillbeselectedforoptimalseparationanddetectionofthetargetanalytes.
-Multiplereactionmonitoring(MRM)modewillbeusedforthequantificationofHCAs.
-Calibrationcurveswillbeconstructedusingstandardsolutionsofthe16HCAstodeterminethelinearity,sensitivity,andlimitsofdetection(LODs)andquantification(LOQs)ofthemethod.
ResultsandDiscussion:
-Theperformanceofeachpre-processingmethodwillbeevaluatedbasedontherecovery,sensitivity,reproducibili