一种检测亚硫酸氢根的苯并咪唑类荧光增强型探针及其实际应用（英文）-豆柴文库

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一种检测亚硫酸氢根的苯并咪唑类荧光增强型探针及其实际应用（英文）
Title:ABenzoimidazole-basedFluorescence-enhancedProbeforDetectionofBisulfiteandItsPracticalApplication
Introduction:
Bisulfiteisanimportantsubstancethatiswidelyusedinvariousfieldssuchasfoodpreservation,pharmaceuticalindustry,anddisinfection.However,excessiveintakeofbisulfitecanleadtoserioushealthproblems,suchasrespiratorydiseasesandskinirritation.Therefore,thedevelopmentofahighlysensitiveandspecificmethodfordetectingbisulfiteisurgentlyneeded.Fluorescenceprobeshaveattractedmuchattentioninrecentyearsduetotheiradvantagesofhighsensitivity,simplicity,andlowcost.Herein,wereportonthedevelopmentofanovelfluorescence-enhancedprobebasedonbenzoimidazoleforthedetectionofbisulfiteanditspracticalapplication.
Experimental:
Firstly,thebenzoimidazole-basedprobewassynthesizedthroughasimpleone-stepreactionbetween4-(benzo[d]imidazol-2-yl)benzaldehydeand3-aminophenol.Thefluorescencepropertiesoftheprobeanditsinteractionwithbisulfitewerestudiedusingfluorescencespectroscopy.Theresultsshowedthatthefluorescenceoftheprobewassignificantlyenhancedupontheadditionofbisulfiteduetotheformationofabisulfite-inducedring-openingreaction.
Theselectivityoftheprobeforbisulfitewasalsoinvestigatedbyaddingvariousinterferingsubstancessuchasmetalions,anions,andaminoacids.Theresultsindicatedthattheprobeexhibitedhighselectivityforbisulfitewithoutinterferencefromothersubstances.
Toevaluatethepracticalapplicationoftheprobe,wetesteditsperformanceindetectingbisulfiteinrealsamplessuchaswineandjuice.Theresultsshowedthattheprobecouldefficientlydetectbisulfiteinthesesamples,indicatingitspracticalusefulness.
Discussion:
Thebenzoimidazole-basedfluorescence-enhancedprobewedevelopedisahighlysensitiveandselectivemethodfordetectingbisulfite.Theprobeissimpletosynthesizeandeasytooperate,makingitapromisingcandidateforpracticalapplicationindetectingbisulfiteinvariousfields.Thebisulfite-inducedring-openingreactionoftheprobeprovidesauniqueandsensitivesignaltransductionmechanismthatenablestheprobetose