利用高通量测序技术对水稻秸秆中、低温降解菌系的比较分析-豆柴文库

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利用高通量测序技术对水稻秸秆中、低温降解菌系的比较分析
Title:ComparativeAnalysisofLow-TemperatureDegradingBacterialStrainsinRiceStrawusingHigh-ThroughputSequencingTechnology
Abstract:
Ricestraw,anabundantagriculturalwaste,holdsgreatpotentialasavaluablebioresourceforvariousapplications.Biodegradationofricestrawatlowtemperaturesisacrucialstepinitsefficientconversionintobiofuelsandothervalue-addedproducts.Toexplorethediversityandfunctionalpotentialoflow-temperaturedegradingbacterialstrainsinricestraw,weemployedhigh-throughputsequencingtechnology.Thisstudyaimedtoprovideacomprehensiveunderstandingofthemicrobialcommunitycompositionandfunctionalgenesinvolvedinlow-temperaturedegradationofricestraw.
Introduction:
Ricestrawisawidelyavailableagriculturalresidue,primarilyconsistingofcellulose,hemicellulose,andlignin.Efficientutilizationofricestrawisimperativetoreduceenvironmentalpollutionandenhancesustainabilityintheagriculturalsector.Low-temperaturedegradationofricestrawinvolvestheactionofspecificbacterialstrains,whichhaveadaptedtosurviveandthriveincoldenvironments.However,limitedknowledgeexistsregardingthediversityandfunctionalpotentialoflow-temperaturedegradingbacteriainricestraw.
MaterialsandMethods:
Inthisstudy,ricestrawsampleswerecollectedfrompaddyfieldsandsubjectedtometagenomicDNAextraction.High-throughputsequencingwasperformedontheIlluminaplatform,generatingmillionsofDNAsequences.Qualitycontrolanddataprocessingwereconductedusingbioinformaticstools.Taxonomicclassificationwasperformedusingreferencedatabases,andfunctionalgeneannotationwascarriedoutusingspecificdatabasesrelatedtolignocellulosedegradation.
Results:
Theanalysisrevealedadiversebacterialcommunityinthericestrawsample,comprisingseveralbacterialphyla,includingFirmicutes,Proteobacteria,Bacteroidetes,andActinobacteria.Thedominantbacterialgeneraassociatedwithlow-temperaturedegradationofricestrawwereidentified,andtheirrelativeabundancewasdetermined.Functionalgeneanalysiselucidatedthepresenceofgenesencodingcellulases,hemicellulases,andlignin-deg