双水相萃取-高效液相色谱法分析红小豆中酰胺除草剂（英文）-豆柴文库

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双水相萃取-高效液相色谱法分析红小豆中酰胺除草剂（英文）
Title:AnalysisofAcylanilideHerbicidesinRedBeansUsingtheSolid-LiquidExtractionandHigh-PerformanceLiquidChromatographyMethod
Abstract:
Acylanilideherbicideshavebeenwidelyusedinagricultureforweedcontrol.However,theirresiduesinfoodcropssuchasredbeansraiseconcernsduetotheirpotentialadverseeffectsonhumanhealth.Therefore,areliableandsensitivemethodisneededfortheanalysisofacylanilideherbicidesinredbeans.Inthisstudy,asolid-liquidextraction(SLE)combinedwithhigh-performanceliquidchromatography(HPLC)methodwasdevelopedforthedeterminationofacylanilideherbicidesinredbeans.Themethodwasvalidatedforlinearity,accuracy,precision,andlimitsofdetection(LOD)andquantification(LOQ).Thedevelopedmethodwassuccessfullyappliedtoanalyzesixcommonlyusedacylanilideherbicidesinredbeans.
1.Introduction:
Acylanilideherbicidesareaclassofsyntheticcompoundswidelyusedforweedcontrolinagriculture.Theyhavebecomepopularduetotheirbroad-spectrumactivity,lowtoxicitytomammals,andresistancetodegradation.However,theirpersistenceintheenvironmentandpotentialresiduesinfoodcropshaveraisedconcernsabouttheirimpactonhumanhealth.Redbeansareapopularfoodcropconsumedworldwideandtheircontaminationwithacylanilideherbicidesneedstobemonitored.Therefore,thedevelopmentofareliableandsensitiveanalysismethodisessentialforthequantificationofacylanilideherbicidesinredbeans.
2.ExperimentalMethods:
2.1ReagentsandMaterials:
Thesixacylanilideherbicides(A,B,C,D,E,F)wereobtainedfromareputablesupplier.HPLC-gradesolventsandchemicals,includingacetonitrile,methanol,andwater,wereusedforthestudy.Redbeansampleswereobtainedfromlocalmarkets.
2.2SamplePreparation:
Theredbeansamplesweregroundtoafinepowderusingablenderandstoredinarefrigeratoruntilanalysis.Anaccuratelyweighedportionofthesamplewasextractedwithacetonitrileusingsolid-liquidextraction.TheextractwasthenpurifiedusingaC18cartridgeandevaporatedtodrynessundernitrogengas.
2.3HPLCAnalysis:
ThedriedresiduewasreconstitutedinmethanolandsubjectedtoHPLCanalysisusingaC18column.A