基于时域荧光寿命的水体溶解氧浓度检测方法-豆柴文库

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基于时域荧光寿命的水体溶解氧浓度检测方法
Abstract:
Dissolvedoxygen(DO)concentrationplaysavitalroleintheaquaticecosystemasitaffectsthesurvivalandmetabolismofaquaticorganisms.Therefore,accuratedeterminationofDOconcentrationinwaterbodiesisessential,andavarietyofmethodshavebeendevelopedforthispurpose.Inthispaper,wereviewtheuseoftime-resolvedfluorescencelifetime-basedmethodsforDOconcentrationdetectioninwaterbodies.Thistechniquehasbeenwidelyusedduetoitshighsensitivityandspecificity,non-invasivenature,andtheabilitytorapidlymeasureDOconcentrationinrealtime.Thepaperprovidesanoverviewoftheprinciples,instrumentation,andapplicationsoftime-resolvedfluorescencelifetime-basedmethodsforDOconcentrationdetectioninwaterbodies.
Introduction:
Dissolvedoxygenconcentrationisacriticalparameterintheaquaticecosystemasitplaysasignificantroleinthesurvivalandmetabolismofaquaticorganisms.DOconcentrationaffectsvariousparameterssuchaswatercirculationandnutrientcyclinginaquaticecosystems.TheassessmentofDOconcentration,therefore,isimportantinecologicalresearch,waterqualityanalysis,andenvironmentalmonitoring.Inaquaticenvironments,DOconcentrationcanbeaffectedbyvariousfactorssuchastemperature,pressure,andsalinity.Thus,itiscrucialtomeasureDOconcentrationaccuratelyandrapidlyinreal-timetoaddressvariousenvironmentalissues,suchashypoxia,eutrophication,andharmfulalgalblooms.SeveralconventionalmethodshavebeenusedforDOmeasurement.However,thesemethodshavesomelimitationssuchastheirversatilityandsensitivity.Hence,thereisaneedtodevelopmorerobustandefficientmethodsthatcanprovidereliableandaccuratedata.
Time-resolvedfluorescencelifetime-basedmethodshavebeenwidelyusedtodetermineDOconcentrationinwaterenvironments.Theprinciplebehindthistechniqueissimpleandinvolvesmeasuringthefluorescencelifetimeofanopticalprobeexcitedbyalightsource.ThefluorescencelifetimeoftheprobeissensitivetoDOconcentration,andhencethedeterminationofDOconcentrationisbasedonchangesinfluorescencelifetime.Thisapproachisnon-invasiveanddoesnotrequirephysicalcontactwiththesam