基于纳米锥森林的三维SERS基底检测miRNA（英文）-豆柴文库

您所在位置：网站首页 / 基于纳米锥森林的三维SERS基底检测miRNA（英文）.docx / 文档详情

在线预览结束，喜欢就下载吧，查找使用更方便

5 金币

下载文档

/ 2

下载提示

如果您无法下载资料，请参考说明：

1、部分资料下载需要金币，请确保您的账户上有足够的金币

2、已购买过的文档，再次下载不重复扣费

3、资料包下载后请先用软件解压，在使用对应软件打开

文本预览

基于纳米锥森林的三维SERS基底检测miRNA（英文）
Title:Three-DimensionalSERSSubstrateformiRNADetectionBasedonNanopillarForest
Abstract:
Surface-enhancedRamanscattering(SERS)hasemergedasapowerfulanalyticaltechniquefordetectingtraceanalytes,includingmicroRNAs(miRNAs),duetoitshighsensitivityandselectivity.Inthisstudy,wepresentanovelthree-dimensionalSERSsubstratebasedonananopillarforestformiRNAdetection.Thesubstratewasfabricatedusingacombinationofnanoimprintlithographyandmetaldepositiontechniques.TheSERSenhancementpropertiesofthesubstratewereoptimizedthroughsystematicinvestigationofthesize,shape,anddensityofthenanopillars.Inaddition,themiRNAdetectionperformanceoftheSERSsubstratewasevaluatedbyimmobilizingcomplementaryDNAprobesontothenanopillarsurface.Theresultsdemonstratethatthedevelopedthree-dimensionalSERSsubstrateoffershighsensitivityandexcellentreproducibilityforthedetectionofmiRNA.
1.Introduction:
MicroRNAs(miRNAs)areshortnon-codingRNAsthatplaycrucialrolesintheregulationofgeneexpression.AbnormalexpressionlevelsofspecificmiRNAshavebeencorrelatedwithvariousdiseases,includingcancer,cardiovasculardiseases,andneurodegenerativedisorders.Therefore,thedevelopmentofsensitiveandreliablemiRNAdetectionmethodsisofgreatimportanceforbothbasicresearchandclinicaldiagnostics.Surface-enhancedRamanscattering(SERS)hasdemonstratedoutstandingcapabilitiesformiRNAdetectionduetoitsultrasensitivityandmolecularspecificity.However,traditionalplanarSERSsubstrateshavelimitationsintermsofreproducibilityandsensitivity.Theintroductionofthree-dimensionalSERSsubstrates,suchasnanopillarforests,hasshowngreatpromiseinovercomingtheselimitations.
2.FabricationofThree-DimensionalSERSSubstrate:
Thefabricationofthethree-dimensionalSERSsubstrateinvolvedatwo-stepprocess.First,ananopillarforestwaspreparedbynanoimprintlithography,whichenabledprecisecontroloverthesize,shape,anddensityofthenanopillars.Then,athinmetalliclayer(e.g.,goldorsilver)wasdepositedontothenanopillarsurface,providingSERSenhancement.Thethicknessofthemetallayerwasop