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杂醇油酶法制备天然等同酯香料的研究 Title:ResearchontheProductionofNaturalEquivalentEstersFragrancesviatheEsterificationofMixedAlcohols Abstract: Thepresentstudyfocusesontheproductionofnaturalequivalentesterfragrancesthroughtheesterificationofmixedalcoholsmethod.Thesynthesisoffragranceestershasgainedincreasingattentionintheflavorandfragranceindustryduetothedemandfornaturalandsustainablealternatives.Thispaperexplorestheapplicationofmixedalcoholsasarawmaterialforthesynthesisofesterfragrancesanddiscussesthekeyfactorsaffectingtheesterificationprocess.Theresultsdemonstratethattheesterificationofmixedalcoholsusinglipaseasacatalystcanproducenaturalequivalentesterfragranceswithenhancedaromaprofiles. 1.Introduction: 1.1Background:Theincreasingdemandfornaturalandsustainablealternativesinthefragranceindustryhasledtotheexplorationanddevelopmentofnewmethodsforproducingesterfragrances. 1.2Objectives:Thisstudyaimstoinvestigatethefeasibilityofusingtheesterificationofmixedalcoholsmethodfortheproductionofnaturalequivalentesterfragrances. 1.3Significance:Thedevelopmentofnaturalequivalentesterfragrancesnotonlysatisfiesconsumerdemandsbutalsoreducesdependenceonsyntheticfragrancecompounds. 2.MaterialsandMethods: 2.1RawMaterials:Mixedalcoholsobtainedfromnaturalsourceswereusedastheprimaryrawmaterial,includingamixtureofvariousstraight-chainandbranched-chainalcohols. 2.2EnzymaticCatalyst:Lipase,acommonbiocatalystwidelyusedforesterificationreactions,wasselectedasthecatalyst. 2.3EsterificationReaction:Theprocedureinvolvedtheadditionofrawmaterials,catalyst,andsolventinareactor,followedbyreactionundercontrolledconditionsoftemperature,pressure,andtime. 2.4Characterization:Thesynthesizedesterfragranceswerecharacterizedusingvariousanalyticaltechniques,includinggaschromatography-massspectrometry(GC-MS)andsensoryevaluation. 3.ResultsandDiscussion: 3.1EffectofSubstrateConcentration:Theesterificationreactiondemonstratedanoptimumsubstrateconcentrationformaximumyieldofesterfragrances. 3.2InfluenceofCatalystConcentration:Th

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