病毒感染海洋球石藻Emiliania huxleyi的转录组分析-豆柴文库

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病毒感染海洋球石藻Emilianiahuxleyi的转录组分析
Introduction
Emilianiahuxleyi(Eh)isacoccolithophore,commonlyfoundintheoceansworldwide.Itplaysacriticalroleinthemarinefoodwebandglobalcarboncycling.However,Ehfrequentlysuffersfromviralinfections,causingsignificantmortalityandecologicalimpact.Thus,substantialeffortshavebeenputintoelucidatingtheinteractionbetweenEhanditsviruses,especiallyatthemolecularlevel.Inthisstudy,weaimtoanalyzethetranscriptomechangesofEhafterviralinfection,sheddinglightontheunderlyingmolecularmechanisms.
Methods
EhcellswereinoculatedwithEhV-201,assDNAvirusthatinfectsEh.TotalRNAwasextractedfrombothinfectedandnon-infectedcellsatdifferenttimepointspost-infection(0,6,12,and24hours),followedbysequencingusingIlluminaHiSeq2000.ThecleanreadsweremappedtotheEhgenomeusingTopHat2,andtheexpressionlevelsofgeneswerecalculatedusingCufflinks.Differentiallyexpressedgenes(DEGs)wereidentifiedusingacutoffoffalsediscoveryrate(FDR)<0.05andfoldchange(FC)>2.Geneontology(GO)andKyotoEncyclopediaofGenesandGenomes(KEGG)pathwayanalyseswereperformedusingKOBAS2.0.
Results
Atotalof16,105genesweredetectedatleastinonesample,and12,935geneswerecommonlyexpressedinallsamples.Amongthem,1,205genesweresignificantlydifferentiallyexpressedafterviralinfection,with691upregulatedand514downregulatedgenes.ThenumberofDEGsincreasedwithtime,reachingthemaximumat24hourspost-infection(930genes).GOanalysisshowedthattheupregulatedgeneswereenrichedinimmunesystem-relatedpathways,suchasdefenseresponse,toll-likereceptorsignalingpathway,andchemokinesignalingpathway.Meanwhile,thedownregulatedgeneswereenrichedinthecarbonfixationpathway,photosynthesis,andDNAreplicationpathways.KEGGpathwayanalysisfurtherconfirmedthesignificantenrichmentofimmune-relatedpathwaysintheupregulatedgenes.
Discussion
OurresultssuggestthatviralinfectiontriggersarobustimmuneresponseinEh,involvingactivationofvariousimmunesignalingpathways.ThisfindingisconsistentwithpreviousstudiesthatEhrespondstoviralinfectionbyproducingreactiveoxygenspecies,heatshockprotei