超临界流体色谱法快速定量分析吴茱萸中的吴茱萸碱和吴茱萸次碱（英文）-豆柴文库

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超临界流体色谱法快速定量分析吴茱萸中的吴茱萸碱和吴茱萸次碱（英文）
Title:RapidQuantitativeAnalysisofWuZhuYuAlkaloidsandWuZhuYuSubalkaloidsinFructusEvodiaeUsingSupercriticalFluidChromatography
Abstract:
Supercriticalfluidchromatography(SFC)isapowerfultechniquethathasgainedsignificantattentioninrecentyearsduetoitsabilitytoseparateandquantifyvariouscompoundsquickly.ThisstudyaimedtodeveloparapidSFCmethodforthequantitativeanalysisofWuZhuYualkaloidsandWuZhuYusubalkaloidsinFructusEvodiae.
Introduction:
FructusEvodiae,commonlyknownasWuZhuYu,isatraditionalChinesemedicinalherbthathasbeenusedforitsvarioustherapeuticbenefits,includingpainrelief,vasodilation,anti-inflammatory,andanticanceractivities.ThemainbioactivecompoundsfoundinWuZhuYuarethealkaloidsandsubalkaloids,suchasevodiamineandrutaecarpine.ThesecompoundsexhibitsignificantpharmacologicalactivitiesandareoftenemployedasmarkersforqualitycontrolofFructusEvodiae.Therefore,arapidandreliableanalyticalmethodfortheirquantificationisessential.
Methods:
1.SamplePreparation:
-WuZhuYusamplesweregroundintoafinepowder.
-Aknownamountofthepowderedsamplewasaccuratelyweighedandextractedusinganappropriatesolvent(e.g.,methanol).
-Theextractwasthenfilteredandevaporatedunderreducedpressuretoobtainaresidue.
2.Instrumentation:
-SFCanalysiswasperformedonahigh-performanceSFCsystemequippedwithaUVdetector.
-Theanalyticalcolumnusedwaspackedwithsuitablestationaryphasematerials,suchassilicagel.
-ThemobilephaseconsistedofsupercriticalCO2asthemaincomponent,alongwithasuitablemodifiertoenhancetheseparationefficiency.
3.MethodDevelopment:
-OptimizationoftheSFCconditions,includingcolumntemperature,pressure,modifierconcentration,andflowrate,wasconductedtoachievethebestseparationandpeakresolutionforWuZhuYualkaloidsandsubalkaloids.
-AsuitablewavelengthfordetectionofthetargetcompoundswasselectedbasedontheirUVabsorbancespectra.
4.MethodValidation:
-ThedevelopedSFCmethodwasvalidatedforlinearity,accuracy,precision,LOD/LOQ,androbustnessaccordingtoappropriateguidelines.
-WuZhuYusamplesofknownconcen