高效液相色谱法测定畜禽肉中8种生物胺含量国家标准方法的改进-豆柴文库

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高效液相色谱法测定畜禽肉中8种生物胺含量国家标准方法的改进
Title:ImprovementsintheNationalStandardMethodfortheDeterminationofEightBiogenicAminesinMeatandPoultrybyHighPerformanceLiquidChromatography
Abstract:
Biogenicaminesarenaturalcompoundsthatareformedthroughthedecarboxylationofaminoacidsinanimaltissues.Highlevelsofbiogenicaminesinmeatandpoultryproductsindicatethepresenceofmicrobialcontaminationandcanposesignificanthealthriskstoconsumers.Inordertoensurefoodsafety,itisvitaltodeveloprobustandaccuratemethodsforthedeterminationofbiogenicaminesinmeatandpoultry.Thispaperaimstoproposeimprovementsinthecurrentnationalstandardmethodforthedeterminationofeightbiogenicaminesinmeatandpoultryusinghighperformanceliquidchromatography(HPLC).
1.Introduction:
1.1Backgroundonbiogenicaminesandtheirsignificanceinmeatandpoultryproducts.
1.2Importanceofaccuratedeterminationofbiogenicaminesinmeatandpoultry.
1.3Overviewofthecurrentnationalstandardmethodanditslimitations.
2.Methodology:
2.1Principlesofhighperformanceliquidchromatography(HPLC)anditsapplicationintheanalysisofbiogenicamines.
2.2Comparisonofdifferentextractiontechniquesforbiogenicaminesfrommeatandpoultrysamples.
2.3Optimizationofchromatographicconditions,includingcolumnselection,mobilephasecomposition,andflowrate.
2.4Samplepreparation,includingderivatizationtechniquestoenhancedetectionsensitivityandimproveseparationefficiency.
2.5Validationoftheimprovedmethod,includingtheevaluationoflinearity,limitofdetection,limitofquantification,accuracy,precision,androbustness.
3.ResultsandDiscussion:
3.1Comparisonofthecurrentnationalstandardmethodwiththeimprovedmethod.
3.2Evaluationoftheimprovedmethodthroughtheanalysisofrealmeatandpoultrysamples.
3.3Discussionoftheimprovementsmadeandtheirimpactontheaccuracy,sensitivity,andreliabilityoftheanalysis.
3.4Comparisonoftheproposedmethodwithotherreportedmethodsforthedeterminationofbiogenicaminesinmeatandpoultry.
4.Conclusion:
4.1Summaryoftheimprovementsmadetothenationalstandardmethod.
4.2Significanceoftheproposedmethodinensurin