响应面法优化苦荞麸皮粉蛋白质提取工艺-豆柴文库

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响应面法优化苦荞麸皮粉蛋白质提取工艺
Title:OptimizationofProteinExtractionfromBuckwheatBranFlourUsingResponseSurfaceMethodology
Abstract:
Buckwheatbranisarichsourceofbioactivecompounds,includingproteins,whichhavevarioushealthbenefits.However,theextractionofproteinsfrombuckwheatbranflourcanbechallengingduetothepresenceofanti-nutritionalfactorsandcomplexcellwallstructures.Thisstudyaimedtooptimizetheproteinextractionprocessfrombuckwheatbranflourusingresponsesurfacemethodology(RSM).RSMinvolvesdesigningexperiments,fittingmathematicalmodels,andoptimizingvariablestoobtainthedesiredresponse.Theeffectsofthreeindependentvariables,namelyextractiontemperature,extractiontime,andsolvent-to-sampleratio,onproteinextractionyieldwereinvestigatedusingaBox-Behnkendesign.Theresultsshowedthatthehighestproteinextractionyield(X%)wasachievedatanextractiontemperatureofT°C,extractiontimeoftmin,andsolvent-to-sampleratioofR.
1.Introduction
1.1Background
Buckwheat(Fagopyrumesculentum)isapseudo-cerealcropthatiswidelycultivatedforitsnutritionalandhealthbenefits.Buckwheatbran,theouterlayerofthegrain,isavaluablebyproductthathasbeenextensivelystudiedforitshighproteincontent.Proteinextractionfrombuckwheatbranflourcanyieldahigh-qualityproductwithpotentialapplicationsinvariousfoodandpharmaceuticalindustries.
1.2Objectives
ThemainobjectiveofthisstudywastooptimizetheproteinextractionprocessfrombuckwheatbranflourusingRSM.Thespecificobjectivesincludeddeterminingtheeffectsofextractiontemperature,extractiontime,andsolvent-to-sampleratioonproteinextractionyieldandoptimizingthesevariablestomaximizetheproteinyield.
2.MaterialsandMethods
2.1Materials
Buckwheatbranflourwasobtainedfromalocalsupplier.Chemicalsandsolventswereofanalyticalgradeandwereusedwithoutfurtherpurification.
2.2Proteinextractionprocedure
Theproteinextractionprocedureinvolvedseveralsteps,includingsamplepreparation,extraction,andquantificationofproteincontent.Theextractionwascarriedoutusingaspecificsolventwithvaryingextractiontemperature,extractiontime,andsolvent-t