Rabies virus nucleoprotein as a carrier for-豆柴文库

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RabiesvirusnucleoproteinasacarrierforforeignantigensMartinL.Koser*JamesP.McGettigan*GeneS.Tan†MaryEllenSmith†HilaryKoprowski†BernhardDietzschold†andMatthiasJ.Schnell*‡Departmentsof*BiochemistryandMolecularPharmacologyand†MicrobiologyJeffersonMedicalCollegeThomasJeffersonUniversityPhiladelphiaPA19107-5541ContributedbyHilaryKoprowskiMay182004Rabiesvirus(RV)nucleoprotein(N)tightlyencapsidatesthePartoftheimmuneprotectionfrompathogensrequiresatgenomicandantigenomicRNAofRVtoformtheviralribonucle-leastastrongBcell-mediatedhumoralresponse.Ithasbeenoprotein(RNP)complex.AntigenssuchasNpresentedinahighlypreviouslyshownwithdifferentvirus-basedexpressionsystemsorganizedstructurearesufﬁcientandevendesirabletoactivateBthatavarietyofviralparticlesorvirus-likeparticlescanserveascellstoproliferateandproduceantibodies.Inadditiontoactivat-carriersofforeignBcellepitopeswithintheviralcapsidoringBcellstoproliferateithasbeenshownthatRVNintheRNPenvelopeproteinoftheviralparticle.TheseepitopecarrierscomplexinducespotentThelpercellresponsesresultinginlong-resultedinstrongBcellresponsesagainsttheexpressedforeignlastingandstronghumoralimmuneresponsesagainstRV.Theepitopesindicatingausefulnessofcarrierproteins(14–20).possibilitytosystematicallyincorporateforeigngenesintotheHowevermostoftheseapproachesarerestrictedtoincorpora-genomeofRVandproducearecombinantvirusallowsustotionofonlyshortpeptidesthatdefineepitopesduetotheexaminewhethertheimmunogenicityofforeignantigenscanberestrictedcloningcapacitywithinthecarrierproteins.Wehy-enhancedbyincorporationintotheRVRNPstructure.TotestthispothesizedthatRVNasacarrierproteinforforeignepitopeshypothesisweconstructedarecombinantRVexpressingaRVmightcircumventthisproble