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第页共NUMPAGES3页 金枪鱼肉降解用高产蛋白酶菌株的筛选及鉴定 【摘要】:^p:采用透明圈法从舟山普陀白山土壤中筛选出3株能分泌胞外蛋白酶的菌株,并对蛋白酶活力最高的菌株进行分子鉴定及最适宜生长条件分析^p。选择福林酚试剂法测定其蛋白酶活力,对蛋白酶活力最高的菌株进行16SrRNA基因测序,用MEGA软件构建系统发育树,并考察其接种量、温度、时间对该菌株产酶活力的影响。结果表明,zjoub-1菌为高产蛋白酶菌属,并确定为枯草芽孢杆菌。该菌株用于金枪鱼暗色肉发酵时的最适宜条件为接种量2%、pH7.4、温度37℃、时间48h。在此生长条件下,菌株产蛋白酶活力最高,为49.21U/mL,能够有效地降解金枪鱼暗色肉。 【关键词】:^p:金枪鱼肉;透明圈法;福林酚试剂法;筛选;鉴定;生长条件 中图分类号:S965.332文献标识码:A文章编号:0439-8114(20________)13-2506-06 DOI:10.14088/jki.issn0439-8114.20________.13.027 ScreeningandIdentificationofAStrainwithHighProteaseinFermentationofTunaDarkMeat WULi-na1,ZHAO____iao-hui1,GUOKai-qing1,YANGYu-jie1,FANG____u-bo1,2,CHEN____iao-e1,2,ZHANGYin-zhao3 (1.CollegeofFoodandMedicine,ZhejiangOceanUniversity,Zhoushan316022,Zhejiang,China;2.StateKeyLaboratoryofAquaticProductsProcessingofZhejiangProvince,Zhoushan316022,Zhejiang,China;3.ZhejiangFengyuBiologicalProductsLimitedCompany,Zhoushan316104,Zhejiang,China) Abstract:UsingThreestrainsofbacteriasecretinge____tracellularproteasewereisolatedfromsoilofBaishaninZhoushanbytransparentcirclemethod,thebacteriumwithhighestproteaseactivitywasidentifiedandthemostsuitablegrowthconditionswasstudied.ThecollectedsleswerescreenedbytransparentcirclemethodtogetthreepurestrainswhoseproteaseactivitywouldbemeasuredbyForintphenolreagentmethod.Thestrainwiththehighestproteaseactivitywasidentifiedbythe16SrRNAsequenceanalysisandthephylogeictreewasconstructedbyMEGAsoftware.Theinoculationamount,temperature,timeandpHhadbeenanalyzedontheinfluenceofitsproteaseactivity.Theresultsshowedthatthestudydemonstratesthatthezjoub-1strainwithhighestproteaseactivitywasdefinedasbacillussubtilis.TheoptimalconditionforfermentationofTunadarkmeatwasasfollows:theinoculationquantitywas2%,theincubationtemperaturewas37℃,theincubationtimewasabout48h,theinitialpHwas7.4.Underthiscircumstance,thestrainhasthehighestproteaseactivity,whichillustratesthatthestrainwithhighproteaseactivitycandegradetheTunadarkmeatefficiently.Keywords:Tunadarkme(

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