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InterferenceFootprinting“Chromatinisthelastrefugeofscoundrels”AMitoticHumanChromosomeChromatinBasicUnitofChromatinStructure:PolynucleosomeLimitedMicrococcalNulceaseDigestionof
ChromatinGeneratesa~200bpLadderH1The30nmChromatinFiberAMitoticHumanChromosomeNuclearScaffold/MatrixTheNucleosome
146bpDNA		2XH3,H4,H2A,H2BIdentities=74/82(90%),Positives=79/82(96%)

Query:22ILRDNIQGITKPAIRRLARRGGVKRISGLIYEEVRAVLKSFLESVIRDSVTYTEHAKRKT81
+LRDNIQGITKPAIRRLARRGGVKRISGLIYEERVLKFLE+VIRD+VTYTEHAKRKT
Sbjct:21VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKT80

Query:82VTSLDVVYALKRQGRTLYGFGG103
VT++DVVYALKRQGRTLYGFGG
Sbjct:81VTAMDVVYALKRQGRTLYGFGG102MWChromosomalProteinsDomainStructureofHistonesH2BH34helix-bundle4helix-bundleH4H4H4H4HistoneCoreH2BH2BN-term
helixN-term
helixEnergeticandTopological
ConsequencesofDNAWrappingBasepair:Basepair:Basepair:19AreNucleosomes“Positioned?”ImplicationsofTranslationalPositioningN-ARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTE-H3H4H4H4N-SGRGKGGKGLGKGGAKRHRKVLRDNIQ-H4???The“Linker”HistoneH1/H5HistoneH5reducessaltrequirement
forchromatincompactionHelixIII-
Majorgroove
bindingNTheSolenoidModelforthe30nmFiberThe30nmChromatinFiberCondensins