猪瘟和蓝耳病疫苗中牛病毒性腹泻病毒污染情况的检测与分析毕业论文.docx

青岛农业大学毕业论文题目：猪瘟和蓝耳病疫苗中牛病毒性腹泻病毒污染情况的检测与分析姓名：南76767学院：动物科技学院专业：动物医学专业班级：1003学号：20103637指导教师：韩先杰2014年5月21日目录中文摘要······································································································IAbstract··························································································II引言································································································2TOC\o"1-3"\h\z\uHYPERLINK\l"_Toc389764242"1材料与方法PAGEREF_Toc389764242\h2HYPERLINK\l"_Toc389764243"1.1试验材料PAGEREF_Toc389764243\h2HYPERLINK\l"_Toc389764244"1.1.1试验疫苗PAGEREF_Toc389764244\h2HYPERLINK\l"_Toc389764245"1.1.2试验试剂PAGEREF_Toc389764245\h2HYPERLINK\l"_Toc389764246"1.1.3试验主要仪器设PAGEREF_Toc389764246\h2HYPERLINK\l"_Toc389764247"1.2试验方法PAGEREF_Toc389764247\h3HYPERLINK\l"_Toc389764248"1.2.1RNA的提取PAGEREF_Toc389764248\h3HYPERLINK\l"_Toc389764249"1.2.2RT-PCR检测BVDVPAGEREF_Toc389764249\h3HYPERLINK\l"_Toc389764250"1.2.3巢式PCR扩增PAGEREF_Toc389764250\h4HYPERLINK\l"_Toc389764251"1.2.4琼脂糖凝胶电泳PAGEREF_Toc389764251\h4HYPERLINK\l"_Toc389764252"1.2.5胶回收提纯PAGEREF_Toc389764252\h4HYPERLINK\l"_Toc389764253"1.2.6回收产物连接PAGEREF_Toc389764253\h5HYPERLINK\l"_Toc389764254"1.2.7转化反应PAGEREF_Toc389764254\h5HYPERLINK\l"_Toc389764255"1.2.8阳性克隆筛选PAGEREF_Toc389764255\h6HYPERLINK\l"_Toc389764256"1.3序列分析PAGEREF_Toc389764256\h6HYPERLINK\l"_Toc389764257"2结果与分析PAGEREF_Toc389764257\h7HYPERLINK\l"_Toc389764258"2.1PCR扩增结果PAGEREF_Toc389764258\h7HYPERLINK\l"_Toc389764259"2.1.1巢式PCR扩增结果PAGEREF_Toc389764259\h7HYPERLINK\l"_Toc389764260"2.1.2菌液PCR结果PAGEREF_Toc389764260\h8HYPERLINK\l"_Toc389764261"2.2测序结果PAGEREF_Toc389764261\h9HYPERLINK\l"_Toc389764262"2.3序列分析PAGEREF_Toc389764262\h11HYPERLINK\l"_Toc389764263"2.3.1进化树分析PAGEREF_Toc389764263\h11HYPERLINK\l"_Toc389764264"2.3.2同源性比较PAGEREF_Toc389764264\h12HYPERLINK\l"_Toc389764266"3讨论PAGEREF_Toc389764266\h13HYPERLINK\l"_Toc3